Delivery of chondroitinase ABC and glial cell line-derived neurotrophic factor from silk fibroin conduits enhances peripheral nerve regeneration.
Identifieur interne : 000046 ( Main/Exploration ); précédent : 000045; suivant : 000047Delivery of chondroitinase ABC and glial cell line-derived neurotrophic factor from silk fibroin conduits enhances peripheral nerve regeneration.
Auteurs : Wesley N. Sivak [États-Unis] ; James D. White [États-Unis] ; Jacqueline M. Bliley [États-Unis] ; Lee W. Tien [États-Unis] ; Han Tsung Liao [États-Unis] ; David L. Kaplan [États-Unis] ; Kacey G. Marra [États-Unis]Source :
- Journal of tissue engineering and regenerative medicine [ 1932-7005 ] ; 2017.
Abstract
Nerve conduits are a proven strategy for guiding axon regrowth following injury. This study compares degradable silk-trehalose films containing chondroitinase ABC (ChABC) and/or glial cell line-derived neurotrophic factor (GDNF) loaded within a silk fibroin-based nerve conduit in a rat sciatic nerve defect model. Four groups of silk conduits were prepared, with the following silk-trehalose films inserted into the conduit: (a) empty; (b) 1 µg GDNF; (3) 2 U ChABC; and (4) 1 µg GDNF/2 U ChABC. Drug release studies demonstrated 20% recovery of GDNF and ChABC at 6 weeks and 24 h, respectively. Six conduits of each type were implanted into 15 mm sciatic nerve defects in Lewis rats; conduits were explanted for histological analysis at 6 weeks. Tissues stained with Schwann cell S-100 antibody demonstrated an increased density of cells in both GDNF- and ChABC-treated groups compared to empty control conduits (p < 0.05). Conduits loaded with GDNF and ChABC also demonstrated higher levels of neuron-specific PGP 9.5 protein when compared to controls (p < 0.05). In this study we demonstrated a method to enhance Schwann cell migration and proliferation and also foster axonal regeneration when repairing peripheral nerve gap defects. Silk fibroin-based nerve conduits possess favourable mechanical and degradative properties and are further enhanced when loaded with ChABC and GDNF. Copyright © 2014 John Wiley & Sons, Ltd.
DOI: 10.1002/term.1970
PubMed: 25424415
Affiliations:
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Sivak</name><affiliation wicri:level="4"><nlm:affiliation>Department of Plastic Surgery, University of Pittsburgh, PA, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Plastic Surgery, University of Pittsburgh, PA</wicri:regionArea><placeName><region type="state">Pennsylvanie</region><settlement type="city">Pittsburgh</settlement></placeName><orgName type="university">Université de Pittsburgh</orgName></affiliation></author><author><name sortKey="White, James D" sort="White, James D" uniqKey="White J" first="James D" last="White">James D. 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Marra</name><affiliation wicri:level="4"><nlm:affiliation>Department of Plastic Surgery, University of Pittsburgh, PA, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Plastic Surgery, University of Pittsburgh, PA</wicri:regionArea><placeName><region type="state">Pennsylvanie</region><settlement type="city">Pittsburgh</settlement></placeName><orgName type="university">Université de Pittsburgh</orgName></affiliation></author></analytic><series><title level="j">Journal of tissue engineering and regenerative medicine</title><idno type="eISSN">1932-7005</idno><imprint><date when="2017" type="published">2017</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Nerve conduits are a proven strategy for guiding axon regrowth following injury. This study compares degradable silk-trehalose films containing chondroitinase ABC (ChABC) and/or glial cell line-derived neurotrophic factor (GDNF) loaded within a silk fibroin-based nerve conduit in a rat sciatic nerve defect model. Four groups of silk conduits were prepared, with the following silk-trehalose films inserted into the conduit: (a) empty; (b) 1 µg GDNF; (3) 2 U ChABC; and (4) 1 µg GDNF/2 U ChABC. Drug release studies demonstrated 20% recovery of GDNF and ChABC at 6 weeks and 24 h, respectively. Six conduits of each type were implanted into 15 mm sciatic nerve defects in Lewis rats; conduits were explanted for histological analysis at 6 weeks. Tissues stained with Schwann cell S-100 antibody demonstrated an increased density of cells in both GDNF- and ChABC-treated groups compared to empty control conduits (p < 0.05). Conduits loaded with GDNF and ChABC also demonstrated higher levels of neuron-specific PGP 9.5 protein when compared to controls (p < 0.05). In this study we demonstrated a method to enhance Schwann cell migration and proliferation and also foster axonal regeneration when repairing peripheral nerve gap defects. Silk fibroin-based nerve conduits possess favourable mechanical and degradative properties and are further enhanced when loaded with ChABC and GDNF. Copyright © 2014 John Wiley & Sons, Ltd.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>Massachusetts</li><li>Pennsylvanie</li></region><settlement><li>Pittsburgh</li></settlement><orgName><li>Université de Pittsburgh</li></orgName></list><tree><country name="États-Unis"><region name="Pennsylvanie"><name sortKey="Sivak, Wesley N" sort="Sivak, Wesley N" uniqKey="Sivak W" first="Wesley N" last="Sivak">Wesley N. Sivak</name></region><name sortKey="Bliley, Jacqueline M" sort="Bliley, Jacqueline M" uniqKey="Bliley J" first="Jacqueline M" last="Bliley">Jacqueline M. Bliley</name><name sortKey="Kaplan, David L" sort="Kaplan, David L" uniqKey="Kaplan D" first="David L" last="Kaplan">David L. Kaplan</name><name sortKey="Liao, Han Tsung" sort="Liao, Han Tsung" uniqKey="Liao H" first="Han Tsung" last="Liao">Han Tsung Liao</name><name sortKey="Marra, Kacey G" sort="Marra, Kacey G" uniqKey="Marra K" first="Kacey G" last="Marra">Kacey G. Marra</name><name sortKey="Tien, Lee W" sort="Tien, Lee W" uniqKey="Tien L" first="Lee W" last="Tien">Lee W. Tien</name><name sortKey="White, James D" sort="White, James D" uniqKey="White J" first="James D" last="White">James D. White</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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